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We use the Millipore Spiral-wound TFF1 and TFF2 cartridges for concentrating 10-20L of insect cell media. They work great, don't cost all that much money, and you don't need the expensive holder they sell. You can use a ring stand and clamp the tubing directly to the cartridge. Our cartridges are several years old and have seen hundreds of liters of media, if you clean them well and pre-clear the media(we use a 10,000xg spin) they will last longer and keep a good flow rate. Nat Nat Clark Graduate Student Garman Lab Biochemistry and Molecular Biology Dept. UMass Amherst On Thu, Mar 24, 2011 at 6:08 PM, Junyu Xiao <[log in to unmask]> wrote: > Dear all, > Sorry for non-crystallography related questions. I am looking for > a Tangential Flow Filtration device to concentrate large volumes of media > (several liters) for protein purification. I am wondering whether I could > get some suggestions on my choices. It appears that both Pall and Millipore > carry this kind of products. I would really like to get some information on > the pros/cons of different models. Any kind feedback will be highly > appreciated. > Best regards, > Junyu > ----------------------------------------------------------- > Junyu Xiao, Ph.D. > University of California, San Diego > Leichtag Room 283 > 9500 Gilman Drive, 0721 > La Jolla, CA 92093-0721 > Lab phone: 858-822-0684 > ------------------------------------------------------------ >