Structures of crosslinked complexes
Dear All,
Does anybody know of examples where labile protein complexes have been crystallized after using non-specific crosslinkers (so excluding complexes with engineered SS bonds, for example)?
Thanks in advance for any examples,
Bert
On 3/23/11 10:19 AM, "Parthasarathy Sampathkumar" <[log in to unmask]">[log in to unmask]> wrote:
Dear Bert,
You could try the program Hole from
http://www.ncbi.nlm.nih.gov/pubmed/9195488
J Mol Graph. <http://www.ncbi.nlm.nih.gov/pubmed/9195488> 1996 Dec;14(6):354-60, 376.
HOLE: a program for the analysis of the pore dimensions of ion channel structural models.
Smart OS <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Smart%20OS%22%5BAuthor%5D> , Neduvelil JG <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Neduvelil%20JG%22%5BAuthor%5D> , Wang X <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Wang%20X%22%5BAuthor%5D> , Wallace BA <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Wallace%20BA%22%5BAuthor%5D> , Sansom MS <http://www.ncbi.nlm.nih.gov/pubmed?term=%22Sansom%20MS%22%5BAuthor%5D> .
-Partha
On Wed, Mar 23, 2011 at 9:02 AM, Van Den Berg, Bert <[log in to unmask]">[log in to unmask]> wrote:
Hello all,
Does anyone know how to get values for pore sizes of membrane channels? I’m not interested in A x B angstrom values measured between atom centers and assuming a regular pore shape, but a “real-life” value of either surface area at the narrowest point or the volume of a block centered on the narrowest point of the pore.
Thanks, Bert