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Regarding adding inhibitors. I'm not sure the effect on crystallization but if you use too much you will get covalent modification of your protein from one of them (I can check my notes as to which one). We searched many MS databases of protein modifications to realize why our protein was the wrong Mr. Then my student happened to mention that he had added 10 times top much. Well at least we now know what not to do. Mark Saper [log in to unmask]