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Hi Angela, My colleagues and I at UCSF published a letter to Clinical Chemistry on this topic some time ago: Canfell, P.C., Binder, S.R. and Khayam-Bashi, H. “Pediatric Reference Intervals for Normetanephrine/Metanephrine (Letter).” Clinical Chemistry 1986; 32:222-223. As you appreciate, this kind of study is very hard to perform, which is why there have been few efforts to repeat it. The method used was GC/MS, which in my hands agreed with HPLC within 15-20%, so this offers some guideline as to what you might expect. The advantage of metanephrines for spot urine is that output and stability do not depend on pH, so I believe you are making the right move. Regards, Steven R. Binder Director, Technology Development Clinical Diagnostics Group Bio-Rad Laboratories 4000 Alfred Nobel Drive Hercules CA 94547 Phone : 510-741-4603 e-mail: [log in to unmask] From: Kremmyda Angela <[log in to unmask]> To: <[log in to unmask]> Date: 03/28/2011 08:32 AM Subject: Urine mets to creatinine ratio Sent by: Clinical biochemistry discussion list <[log in to unmask]> Dear all, We are about to move from measuring urine catecholamines to (urine) metanephrines for screening for phaeo. Currently we ask for spot urine(s) and use the ratio to creatinine and we’d like to keep it like that for the metanephrines (instead of having 24 h urine collections). Our problem is there is no reference range provided by the manufacturer for the ratio and we’re finding it very difficult to find any other data. Are there any users out there that use the ratio to creatinine and would be willing to share their normal range? Our method is HPLC. Any help would be very welcome (we are getting a bit desperate…). Thanks in anticipation Angela Angela Kremmyda Senior Biochemist Dept of Clinical Biochemistry Royal Shrewsbury Hospital Mytton Oak Road Shrewsbury SY3 8XQ Tel: 01743 261158 Fax: 01743 261159 This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in aliance on its contents: to do so is strictly prohibited and may be unlawful. ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/