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Hi Stijn, When you say that I've already defined my groups in my contrast are you referring to the 92 rows and 3 columns relating to group assignments (design matrix)? Does that mean I do not need to demean my data because I'm modeling it in the design matrix? In reference to the 7th contrast Con > Rel, Scz. We've had issues with this interpretation. Previously I've run cluster thresholding and then used the mask from that contrast to pull the % signal change at the subject mean level. Additionally I've tried running my task contrast of interest (let's say A-B) as a group mean and then pulled the % signal change and the subject level. Do you have any suggestions about how to compare 3 groups? Edward H. Patzelt Research Assistant – TRiCAM Lab University of Minnesota – Psychology/Psychiatry VA Medical Center Office: S355 Elliot Hall - Twin Cities Campus Phone: 612-626-0072 Email: [log in to unmask]