2. Purify refolded fraction by gel filtration in FPLC
3. measure total protein after refolding
4. calculate efficiency ...
Why?
I don't see the reason to know the efficiency. If after refolding I would get enough in a defined molecular species,
soluble in a non-denaturing buffer, I would be happy. 'Enough' depends on what you want to do with it.
Some refolding protocols have efficiencies of just 2-5%, but this is enough ...
A.
On Feb 10, 2011, at 10:45, megha goyal wrote:
Hello,
Can anyone let me know how to calculate solubilisation and refolding efficiency. We perform solubilisation and then refolding and check by HPLC if refolding is completed or not [single peak on HPLC]. how do i determine % efficiency of refolding. kindly guide me.
regards,
megha
