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The pH is too low and the EDTA is insoluble. You need to be above pH ~8.0.

Your best bet is to try another chelator.

James

On Feb 21, 2011, at 2:22 PM, Yibin Lin wrote:

> Dear all,
> 
> I got a lot of salt crystals in reservior solution (well solution), which contains 0.1 M phosphate/citrate ph 4.2, PEG200 47%, EDTA-2Na 0-22mM. Reservior solution appears crystals from 12mM EDTA. Could someone help me to explain why?
> 
> Thank you very much!
> 
> Yibin
>