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I assume you want to track down which parts of a crystal you already have exposed ?

I think dithionite leads to brown colouring of the protein after/during exposure. At least we've observed frequently that some ingredients in our crystals turn brown over time and you could clearly see where the beam hit. I don't know how applicable this would be for small beamsizes and a couple of images, but for whole datasets it would work.

James Holton probably has a build-it-yourself solution to this I expect.

Jürgen