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Hi, I was wondering if anyone had possible explanations for a recombinantly expressed soluble protein that runs as 2 equal, slightly overlapping peaks on a cation exhanger but as one peak on a size exclusion column and same electrophoretic mobility on SDS-PAGE. -Ulli Adelaide Ulricke Hain PhD Candidate Johns Hopkins Bloomberg School of Public Health Department of Biochemistry and Molecular Biology 615 North Wolfe Street Baltimore, MD 21205