Dear all, 

I have a question regarding the refinement and density map.

My protein is 261 amino acids long and crystalize very nicely with very high resolution . There is no multiple spot in the image and diffraction looks amazing. I collected a dataset at 1.38 resolution and the space group is P43, overall Rmerg is 0.02 (most likely the space group is P4122, but then the solvent content is less than 16% for one molecule in the assymmetric unit, that are unlikely), so I processed the image in P4 (36% solvent) and run molecular replacement with a model that have 42 sequence identity. I got a solution in P43 that are good enough in some part but in the map there are many gap at even lower sigma level. I tried to refine and Rfree stacked at 36 along with Rwork, which is 33.

I also checked the degradation patteren of the protein in the crystal and it looks not degraded and full length protein crystalize. 

Is there anybody can suggest me anything that I can try?

 

Regards, 

Md. Munan Shaik

PhD Student
Department of Biotehnology
School of Bioscience and Biotechnology
via G. Colombo 03
Padova 35131, Italy
Mobile: 00393275671896
E-mail: [log in to unmask]
             [log in to unmask]