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Hi Juergen Just to correct this - Mosflm can output JPGs, but doesn't read them. On 11 Feb 2011, at 16:35, Bosch, Juergen wrote: <snip> > As an alternative approach I believe Mosflm can use JPG images, so convert your dataset to JPG and remove those spots which belong to the other lattice in Photoshop. This approach might lead you to a "dataset" and provide you with a cell, but I think there are better ways of spending time. > > On Feb 11, 2011, at 11:06 AM, pravinkumar jagtap wrote: > >> Hi to all, >> I have crystals which grow as joint crystals i.e two crystals are joined to one another. I tried to seperate the two and collect dataset but the resolution is not good( probably I am harming the crystals during seperation). Also I collected a dataset with the joined crystals in the loop and I got resolution up to 3.5 A on synchrotron. But when I am trying to index this dataset, the XDS is failing . I also tried to use mosflm but it is also giving warning message that the unit cell parameters are too inaccurate. And this all seems to be because of the two crystals in the loop. Can anyone suggest any strategy which I should take for indexing this dataset. >> >> Thanks, >> Pravin Kumar > Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 0QH