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Dear all, Thanks for your hearty responses. Below is a summary for the information I received. Again, I really appreciate all the help. Best regards, Junyu 1. Yale University, University of Utah, and Wake Forest University are three that have core facilities which perform N-terminal sequencing. 2. A colleague here has had good results with the facility at Iowa state: http://www.biotech.iastate.edu/service_facilities/protein.html If you just want to identify the protein, mass spec may be cheaper. The same place will do that, or we have had excellent results with http://www.appliedbiomics.com/ If you need to confirm N-term construct, Edman degradation is probably still more cost effective. 3. I've gotten decent results from Rockefeller: http://proteomics.rockefeller.edu/edman/edmaninfo 4. We have used Alphalyse (http://www.alphalyse.com/picknpost.html). 5. There is one at the Johns Hopkins University. Synthesis and Sequening facility 6. You can get it here as well... University of Pittsburgh Department of Biological Sciences Fifth and Ruskin Aves Clapp Hall 301 Pittsburgh PA 15260 email: [log in to unmask] ph: 412.624.0106 or Jack Presley Laboratory Manager UC Davis Molecular Structure Facility http://msf.ucdavis.edu (530) 752-7327 7. Proteos 4717 Campus Drive Kalamazoo, Michigan 49008 269.372.3423 http://www.proteos.net ----------------------------------------------------------- Junyu Xiao, Ph.D. University of California, San Diego Leichtag Room 283 9500 Gilman Drive, 0721 La Jolla, CA 92093-0721 Lab phone: 858-822-0684 ------------------------------------------------------------