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type="cite">
I'll swap you for the deamination, as that pertains to lysines...it's
been a long week :)
Steve
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Nadir T. Mrabet
Sent: Friday, February 18, 2011 2:04 PM
To: [log in to unmask]
Subject: Re: [ccp4bb] off-topic: 2 peaks on Cation
Typo! I actually meant deamidation.
Nadir
Pr. Nadir T. Mrabet
Structural& Molecular Biochemistry
Nutrigenex - INSERM U-954
Nancy University, School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33 (0)3.83.68.32.73
Fax: +33 (0)3.83.68.32.79
E-mail: Nadir.Mrabet<at> medecine.uhp-nancy.fr
On 18/02/2011 20:36, Christian Roth wrote:
Hi,
you did not mention anything about your protein, but if it shows a
metal
dependency, than different amounts of the metal ion might changen the
overall
charge and influence the interactions with the ion exchanger. There
might be
also a modification of an aminoacid f.e. decarboxylation of an
aspartate or
glutamate.
Christian
Am Freitag 18 Februar 2011 18:13:36 schrieb Ulli Hain:
Hi, I was wondering if anyone had possible explanations for a
recombinantly expressed soluble protein that runs as 2 equal,
slightly
overlapping peaks on a cation exhanger but as one peak on a size
exclusion
column and same electrophoretic mobility on SDS-PAGE. -Ulli
Adelaide Ulricke Hain
PhD Candidate
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry and Molecular Biology
615 North Wolfe Street
Baltimore, MD 21205
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