Hi Rex, There will be small differences in particular due to the different ways of treating the solvent. How large of a difference are you talking about? Normally the difference should not be very large... And if this related to solvent effects, it will affect the low resolution reflections with a decrease in the effect when you go to higher and higher resolutions (but never completely disappear). Fred. REX PALMER wrote: > Does anyone still use CNS ? > Do we expect Rfree from CNS for example to be different from the value > given by Refmac at the end of the refinement? > > Rex Palmer > > Birkbeck College >