Dear Chandan,

388 buffers are quite a bit. I presume you are talking about intervals of pH and not 388 buffers all at one pH. Can you explain a bit more about how you determined that the buffer is the problem, and what results led you to that conclusion?

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From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of chandan kishore
Sent: Thursday, January 27, 2011 1:43 AM
To: [log in to unmask]
Subject: [ccp4bb] buffers for crystallization

Hi Everyone,

I have to crystallize one protein which contains a zinc fingure motifs, I am facing a problem in crystallization. I already used 388 buffers . Can anyone suggest me some books or papers freely available online on Buffers required for crystallization.
Thanks