Dear ALL;

       Recently I am purifying a cell surface receptor by refolding of its inclusion bodies.

       Actually I can purify some functional monomers of this protein. However, the protein precipitates during the concentration. Interestingly, the precipitates look like crystals but not heavy aggregates.

      I guess the problem is due to the missing of glycosylation. 

      Does anyone have suggestions to increase the current solubility?

     Thanks a lot,

Jerry McCully