Dear ALL;
Recently I am purifying a cell surface receptor by refolding of its inclusion bodies.
Actually I can purify some functional monomers of this protein. However, the protein precipitates during the concentration. Interestingly, the precipitates look like crystals but not heavy aggregates.
I guess the problem is due to the missing of glycosylation.
Does anyone have suggestions to increase the current solubility?
Thanks a lot,
Jerry McCully