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Let's say I need to insert two G between resi 98 and 99. I tried in the way as Herman suggest: break the chain in the domain boundary, add terminal residues at the N-terminus of the 2nd domain. But then there is no space to accommodate the inserted residues, the two glycerin backbones overlap with resi 97 and 98. Is there a way to shift the whole 1st domain a bit ?



On Thu, Jan 13, 2011 at 12:16 PM, <[log in to unmask]> wrote:
use coot: calculate - model/fit/refine - add terminal residue.
this will add alanines. you can change these to glycines with the simple mutate option in the same pop up menu.
 
best,
HErman

From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Qing Chen
Sent: Thursday, January 13, 2011 11:58 AM
To: [log in to unmask]
Subject: [ccp4bb] creat a model with insertion

Dear all,

I have the wt protein structure that contains two domains. I want to creat a model with two glycerin inserted between the two domains.
Which software or webserver could do this?
Pls help.


Qing Chen