use coot: calculate - model/fit/refine - add terminal residue. this will add alanines. you can change these to glycines with the simple mutate option in the same pop up menu. best, HErman ________________________________ From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Qing Chen Sent: Thursday, January 13, 2011 11:58 AM To: [log in to unmask] Subject: [ccp4bb] creat a model with insertion Dear all, I have the wt protein structure that contains two domains. I want to creat a model with two glycerin inserted between the two domains. Which software or webserver could do this? Pls help. Qing Chen