I vaguely remember a paper by Aled Edwards, where they did the statistics for their targets at SGC. As far as I recall they engineer with cleavable tag purify and cyrstallize it first with tag and only if they don't get crystals or a structure they cleave off the tag and rerun the screens. I think the numbers where 30% of the proteins have been cleaved.

Jürgen

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Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab:      +1-410-614-4894
Fax:      +1-410-955-3655
http://web.mac.com/bosch_lab/

On Jan 7, 2011, at 9:40 AM, Snijder, Arjan wrote:

Dear all,
 
Best wishes for the new year!
 
I am looking for some statistics about the fraction of protein structures in the pdb that are actually crystallized with a His tag, and from those crystallized with a His tag whether these construct were engineered with a cleavable tag or not. Has anybody done such an analysis and would want to share the outcome?
In the mean time I will mentally prepare myself for re-freshing my perl knowledge to write a script to do such an analysis. Any suggestions on how to quickly analyse this would be appreciated.
 
cheers
 
Arjan
 
Arjan (Harm Jan) Snijder
Associate Principal Scientist
Astra Zeneca R&D, Mölndal
Discovery Enabling Sciences and Capabilities, DECS
SE-431 83 Mölndal Sweden
tel: ++46 31 7763691
 

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