At this resolution it is very much possible to choose a wrong space
group. So try lower symmetry first and see if it helps. Check for
twinning too.
On Sat, 2011-01-08 at 19:08 +0000, Dimitris Ladakis wrote:
> Dear all
> I've got a 3A dataset processed with mosflm and scaled. I've
> runned MOLREP that gave a solution with a score of 0.52. At this
> stage the Rfactor was 44%.
> After my first round of refinement with REFMAC i've got both Rfactor
> and an Rfree at about 43%. After model building and several refinement
> rounds i've got an Rfactor 36% and the RFree is 45%. Since there are
> two molecules in the AU i tried to do NCS phased refinement using
> NCSREF, but it didn't do anything apart from breaking a lot of bonds
> (apparent when looking the model in coot).
> I would greatly appreciate some ideas for improving my model
> Thanks a lot
> D.L
--
"I'd jump in myself, if I weren't so good at whistling."
Julian, King of Lemurs
