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Actually, it is very difficult to say something just based on your simple information. You should give more informations about your work, for example, temperature, cuture, etc. Best, Lin On Fri, Jan 21, 2011 at 8:12 PM, m zhang <[log in to unmask]> wrote: > Dear All, > > Recently I am trying to express a glycoprotein in Drosophila S2 cells. > However, the expression yield is every low and most of the protein expressed > aggregate. I was suggested making new constructs. But I am wondering if > anyone here can give me some suggestions to improve it beyond that since I > am new to glycoprotein? All your input will be greatly appreciated! > > Regards, > > Min >