 |
 |
> Well that is our data on 45 specimen, repun on our MPA. Each time we repan them, they were new specimen compared to their original values. In total we measured 300 separate measurements. About 45 to 50 new specimen each of the following time intervals: 4, 8, 16, 20 and 24 hours. > > I guess this is way we do experiments and not rely on imagination. Perhaps you would like to repeat the experiment yourself and let us know what you find. > > Sincerely > > Elizabeth Mac Namara > Chief Dept. Diagnostic Medicine > Jewish General Hospital > Montreal > > > > > On 2011-01-31, at 9:10 AM, Joel Goodman <[log in to unmask]> wrote: > >> I can’t imagine that re-spinning “within 16 hours” has no significant effect on serum potassium or glucose levels. >> >> Impossible. >> >> If this were the case, why bother to use an SST at all, if testing will be completed within 16 hours of collection? >> >> From: Canadian Society of Clinical Chemists [mailto:[log in to unmask]] On Behalf Of Elizabeth MacNamara >> Sent: Friday, January 28, 2011 2:51 PM >> To: [log in to unmask] >> Subject: Re: Effect of repeat centrifugation >> >> We are hoping to present a poster at the IFCC on the effect of respining primary tubes on potassium. Less than 16 hours it is insignificant, after it has a huge effect. We had to do the study as we often spin our outpatients’ bloods and assay them later. As we use a pre-analytic robotic system we had to know if it was safe or not. >> >> I hope this helps. >> >> Elizabeth Mac Namara >> Dept. Diagnostic Medicine >> Jewish General Hospital >> McGill University, Montreal >> >> From: Canadian Society of Clinical Chemists [mailto:[log in to unmask]] On Behalf Of Andrew Lyon >> Sent: Friday, January 28, 2011 2:27 PM >> To: [log in to unmask] >> Subject: Re: Effect of repeat centrifugation >> >> Hello Dr. Djiana >> >> Most of my experience is anecdotal following investigations of spurious elevated potassium results……and finding staff admit that a specimen was re-centrifuged, (inappropriately). >> >> Re-centrifugation seems to squeeze a little fluid from the compact red cell layer under the gel back into the overlaying plasma…. >> causing unexpected elevated potassium results. I expect you may also see elevation of LD and lactate and possibly phosphate… >> and it may be difficult to find studies about this. >> >> Regards, Andrew >> >> Andrew Lyon, PhD, FCACB, DABCC. >> Clinical Biochemist >> ----------------------------------------------------- >> Calgary Laboratory Services & >> Department of Pathology and Laboratory Medicine, University of Calgary >> Calgary, AB, Canada >> >> Tel: 403.944.3993 >> Fax: 403.944.1364 >> [log in to unmask] >> <image001.gif> >> >> From: Canadian Society of Clinical Chemists [mailto:[log in to unmask]] On Behalf Of Rose Djiana >> Sent: January 28, 2011 12:06 >> To: [log in to unmask] >> Subject: Effect of repeat centrifugation >> >> Hi! >> I need to document the effect of respinning a primary (collection) tubes on any (preferably chemistry) lab test results. >> In the product insert, BD tube simply states: "Tubes should not be re-centrifuged once barrier has formed." >> >> http://www.bd.com/vacutainer/pdfs/blood_collection_tubes_product_insert_VDP40035.pdf >> >> Would you suggest references or did perform a study and would kindly share your findings? >> >> Regards >> >> Rose Djiana Ph.D, CSPQ, FCACBcert >> Clinical Biochemist >> MUHC - Royal Victoria >> 514-934-1934 x 36791 >> [log in to unmask] >> >> >> This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. >> >> >> >> >> ************************************************************************ >> This message, including any attachments, is privileged and may contain confidential information intended only for the person(s) named above. Any other distribution, copying or disclosure is strictly prohibited. If you are not the intended recipient or have received this message in error, please notify us immediately by reply email and permanently delete the original transmission from us, including any attachments, without making a copy. Thank you. >> ************************************************************************ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/