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Hi, Yes, to use your own priors you should use the -A option. If the priors are already aligned with your image then you don't need -a, otherwise you do and it should specify the flirt matrix that would transform your image to the space of the priors. The order of the prior images is CSF, Grey and then White. All the best, Mark On 21 Dec 2010, at 08:18, June L wrote: > Hi I am new to FAST and I need the help with the -A option. > I want to use the customized prior images so I have to use the -A option right? And specify the 3 prior images in order of Grey, White, CSF? And specify the transformation matrix obtained from FLIRT in the -a option? > > Thanks so much for the help > > J >