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Hi, I just started using SPM and have some questions regarding the segmentation of structural images. I did segmentation of my structural image using default settings and got the results as seen in the attached file 'structural_segmented'. The top right image is the original structural image. The bottom image is the normalised image (using seg_sn.mat and the bias corrected image), which of cause is bad due to bad segmentation. I manually reoriented the structural image using "Display" where I changed the values "right, forward, up, pitch, roll and yaw". I then used "Reorient images" and chose my structural image. I did the segmentation on the reoriented image and got results as shown in the attached file 'reoriented_structural_segmented'. The segmented images are still bad. Why do a get such a bad segmentation and what can I do to get a better segmentation? Thanks, Tine