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It should not be a problem providing that the matrices in the image headers do their job properly. Best regards, John On 1 November 2010 16:19, Yannis Paloyelis <[log in to unmask]> wrote: > Dear John/list, > > A rather basic question. Following from some previous posts, I am a bit confused on this point. I have scans that were acquired coronally. With the NewSegment/DARTEL procedure (or the VBM8 procedure) do the native space volumes (T1s) need to actually be saved in the axial orientation (for example, using the very useful code that John posted - reorient5.m) before we begin segmentation or is it sufficient if they can be read "correctly" (i.e. displayed in axial orientation) when opened with "Display" or (Check Reg with the MNI template)? > > I segmented some original images (which showed correctly in display or with Check Reg) and the produced native tissue images (c1 etc) were saved in the original (i.e. coronal) orientation, whereas the rc1 etc. images were saved in the axial orientation. I wonder if this may be a problem later on with DARTEL or not (better find out now!). > > Many thanks in advance, > Yannis >