Hi Matthias,

I suspect the Protein Sequence Assignment table is tied to particular experiment types and will only work for solution NMR experiments at the moment, but Tim, Wayne or Rasmus may know more about this.
At the moment you will probably have do things manually - so for example you can predict the amino acid type for a spin system using the Spin System pop-up and you can assign things directly using the Atom Browser. But I am not aware of you being able to see a string of linked amino acids with assignment options and navigation links anywhere.

Vicky




On 8 November 2010 15:49, Matthias Huber <[log in to unmask]> wrote:
Hi Vicky,

yes, with the right-Mouse menu I can create the links.
How can I make my linked Spin Systems appear in
"Assignment: Protein Sequence Assignment:  Spin System Table" to assign my fragments to the sequence?

Matthias



On Nov 8, 2010, at 2:38 , Vicky Higman wrote:
>
> As regards the sequential links - I have found that e.g. for an NCOCX Analysis seems to pick up of its own accord that the N and CO are i and i-1 to each other (because of the experiment type). However, even if you don't have experiment types, you should be able to set sequential spin systems:
> If you select a peak whose resonances are for example (i+1, i, i) to each other, you can go to the right-Mouse Menu and select "Assign" and then "Set sequential Spin Systems" followed by (F1 +1, F2 0, F3 0). This will then set the two spin systems to be sequential - if you later make an assignment to one, the other will automatically become assigned, too. You should be able to make the sequential assignments you want using this function - I think you could even apply it to a DARR Peak that links two neighbouring residues.
>
> Hope this helps,
>
> Vicky
>



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Dr. Victoria A. Higman-Davies

Dept of Biochemistry
University of Oxford
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