Hi Matthias, I suspect the Protein Sequence Assignment table is tied to particular experiment types and will only work for solution NMR experiments at the moment, but Tim, Wayne or Rasmus may know more about this. At the moment you will probably have do things manually - so for example you can predict the amino acid type for a spin system using the Spin System pop-up and you can assign things directly using the Atom Browser. But I am not aware of you being able to see a string of linked amino acids with assignment options and navigation links anywhere. Vicky On 8 November 2010 15:49, Matthias Huber < [log in to unmask]> wrote: > Hi Vicky, > > yes, with the right-Mouse menu I can create the links. > How can I make my linked Spin Systems appear in > "Assignment: Protein Sequence Assignment: Spin System Table" to assign my > fragments to the sequence? > > Matthias > > > > On Nov 8, 2010, at 2:38 , Vicky Higman wrote: > > > > As regards the sequential links - I have found that e.g. for an NCOCX > Analysis seems to pick up of its own accord that the N and CO are i and i-1 > to each other (because of the experiment type). However, even if you don't > have experiment types, you should be able to set sequential spin systems: > > If you select a peak whose resonances are for example (i+1, i, i) to each > other, you can go to the right-Mouse Menu and select "Assign" and then "Set > sequential Spin Systems" followed by (F1 +1, F2 0, F3 0). This will then set > the two spin systems to be sequential - if you later make an assignment to > one, the other will automatically become assigned, too. You should be able > to make the sequential assignments you want using this function - I think > you could even apply it to a DARR Peak that links two neighbouring residues. > > > > Hope this helps, > > > > Vicky > > > -- **************************************************** Dr. Victoria A. Higman-Davies Dept of Biochemistry University of Oxford South Parks Road Oxford OX1 3QU U.K. E-mail: [log in to unmask] (or [log in to unmask]) http://www.protein-nmr.org.uk ****************************************************