attachments are bad enough, but so far at least we had small ones.
now we have a new record, 2.5 MB!
There are a gadzillion sites in the web to put your image and give us a link to it.
As an additional remark, I would had been tempted to ask the assistant professor in my laboratory to tell me exactly what the hell he did and why he was right
and I was wrong. Asking a couple of thousands of people to possibly tell if a dataset they have not seen or processes might be better or worse than another dataset
they have not seen or processed, is likely counterproductive (see reference 1)
these said, I think you have a crystal with a very long axis, that should had been collected with that axis along the rotation axis, as Mark said.
Very likely, the crystal that the assistant professor worked on had the long axis along the rotation direction and thats why it worked.
Although there is some weird order-disorder issues between planes, my feeling is that you can treat that as more or less a 3d crystal that is imperfect.
A.
==
reference 1:
Percy: You know, they do say that the Infanta's eyes are more beautiful
than the famous Stone of Galveston.
Edmund: Mm! ... What?
Percy: The famous Stone of Galveston, My Lord.
Edmund: And what's that, exactly?
Percy: Well, it's a famous blue stone, and it comes ... from Galveston.
Edmund: I see. And what about it?
Percy: Well, My Lord, the Infanta's eyes are bluer than it, for a start.
Edmund: I see. And have you ever seen this stone?
Percy: (nods) No, not as such, My Lord, but I know a couple of
people who have, and they say it's very very blue indeed.
Edmund: And have these people seen the Infanta's eyes?
Percy: No, I shouldn't think so, My Lord.
Edmund: And neither have you, presumably.
Percy: No, My Lord.
Edmund: So, what you're telling me, Percy, is that something you have never
seen is slightly less blue than something else you have never seen.
Percy: (finally begins to grasp) Yes, My Lord.
On Nov 29, 2010, at 18:14, chen c wrote:
I attached two diffraction images of my crystal, of which one seems normal as protein crystal usually do, while the other one looks very strange ,with very continuous lines on the image.
In fact, of the 180 crystal images diffracted by my crystal, there is a tendency between those two.
I had thought that my crystal is a combination of many two-dimensional crystals, between wich there are translational or rotational translocations, namely resulting in a lack of translational symmetry in the third axes. As a result of this, when I tried to index them using HKL2000, one of the cell parameter is merely several angstroms.
However, of the several data sets from different crystals, one data set is sucessfully indexed by the assistant professor of my laboratory and currently submitted to the operation of Molucular Replacement.
This confused me a lot. Is what I thought wrong? Or is the very crystal that was indexed a special one?
Thanks all
chen
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