It reads like you need to run a lane or two with a positive
control of some kind. Can you grow lysozyme, glucose isomerase, hemoglobin
or other crystals of a protein around the same expected molecular weight and try
run on the gel lanes with about the same amount of crystalline volume as your
putative protein crystals?
Hi everyone,
I have grown some crystals after micro-seeding
starting from thin-small needles from needle-clusters. These crystals are larger
in size than the needles but are comparable to the shape and don't look like
salt crystals. But I cannot see the bands( its a complex) in the SDS-PAGE.I do
not have a home source,handy and would like to send these to the
synchrotron.
Is it possible to NOT see a band of protein crystals in
SDS-PAGE, if, say, the amount of protein is < 1uG?
Has anyone experienced
such a thing (no band in gel, but crystal diffracts)?
It would be nice if I
get observations/suggestions.
ivan