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Hi Sebastiano,

Just to add to Dima's response, I've used ceramic hydroxyapatite column and got great results separating 2 very large membrane proteins complexes that eluted together in DEAE/Q and ran in similar size range in SEC. One eluted in 100-200mM KPi and the the other in 300mM+ KPi ~pH 7.4.

-john



On Mon, Nov 29, 2010 at 9:08 AM, Sebastiano Pasqualato <[log in to unmask]> wrote:
Hi all,
I read/heard that hydroxyapatite column can be used to purify proteins, getting separation results orthogonal to ion exchange and size exclusion chromatography.
I was wondering if any of you would be kind enough to share her/his experience with me, and would suggest vendors and models for such columns.
Thanks in advance,
best,
s

--
Sebastiano Pasqualato, PhD
IFOM-IEO Campus
Dipartimento di Oncologia Sperimentale
Istituto Europeo di Oncologia
via Adamello, 16
20139 - Milano
Italy

tel +39 02 9437 5094
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