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Hi Sebastiano, Just to add to Dima's response, I've used ceramic hydroxyapatite column and got great results separating 2 very large membrane proteins complexes that eluted together in DEAE/Q and ran in similar size range in SEC. One eluted in 100-200mM KPi and the the other in 300mM+ KPi ~pH 7.4. -john On Mon, Nov 29, 2010 at 9:08 AM, Sebastiano Pasqualato < [log in to unmask]> wrote: > Hi all, > I read/heard that hydroxyapatite column can be used to purify proteins, > getting separation results orthogonal to ion exchange and size exclusion > chromatography. > I was wondering if any of you would be kind enough to share her/his > experience with me, and would suggest vendors and models for such columns. > Thanks in advance, > best, > s > > -- > Sebastiano Pasqualato, PhD > IFOM-IEO Campus > Dipartimento di Oncologia Sperimentale > Istituto Europeo di Oncologia > via Adamello, 16 > 20139 - Milano > Italy > > tel +39 02 9437 5094 > fax +39 02 9437 5990 >