Isn't the simplest answer that this is not really an Argand diagram with real and imaginary axes, but simply a diagram showing the graphical addition of the component phasors of the atom's scattering components? And aren't real and imaginary relative terms anyway (in more ways than one, I guess!)? Jacob ----- Original Message ----- From: "William Scott" <[log in to unmask]> To: <[log in to unmask]> Sent: Wednesday, October 13, 2010 12:48 PM Subject: [ccp4bb] embarrassingly simple MAD phasing question Hi Citizens: Try not to laugh. I have an embarrassingly simple MAD phasing question: Why is it that F" in this picture isn't required to be vertical (purely imaginary)? http://www.doe-mbi.ucla.edu/~sawaya/tutorials/Phasing/phase.gif (Similarly in the Harker diagram of the intersection of phase circles, one sees this.) I had a student ask me and I realized that there is this fundamental gap in my understanding. Many thanks in advance. -- Bill William G. Scott Professor Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA 228 Sinsheimer Laboratories University of California at Santa Cruz Santa Cruz, California 95064 USA phone: +1-831-459-5367 (office) +1-831-459-5292 (lab) fax: +1-831-4593139 (fax) ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: [log in to unmask] *******************************************