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Hi Ed, On Thu, Oct 21, 2010 at 02:19:34PM -0400, Ed Pozharski wrote: > > * how the refinement in the lower-symmetry spacegroup is done - since > > there is a real danger (in case it is the high-symmetry spacegroup > > after all) that because of model bias and poorer (independent > > observations)/parameter ratio what seems like a clear single > > conformation is difficult to confirm. > > Absolutely true. As we discussed before, restraining protein copies is > a must as well as maybe some bias removal. As long as the bias removal isn't some brutal simulated-annealing job that shakes everything about - and leads to those few significantly differing sidechains that then convinces us that it really is a lower-symmetry spacegroup. Cheers Clemens -- *************************************************************** * Clemens Vonrhein, Ph.D. vonrhein AT GlobalPhasing DOT com * * Global Phasing Ltd. * Sheraton House, Castle Park * Cambridge CB3 0AX, UK *-------------------------------------------------------------- * BUSTER Development Group (http://www.globalphasing.com) ***************************************************************