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Did you check your data for twinning and/or pseudo symmetry using phenix.xtriage or Pointless? If the space group is incorrect, these programs will also assist you in selecting the correct one. What were the Z-scores for the rotation and translation functions? On Mon, Oct 18, 2010 at 7:39 PM, Jyotica Batra <[log in to unmask]>wrote: > Hi All > > I have a dataset at 1.9A, spacegroup-P212121 (unit cell: 37.7, 39.52, > 231.72, 90, 90, 90), > I used MR phaser and got a structure solution with LLG= 320 (1copy/a.u) . > During refinement, the R-free (50%) and R-factors (42%) never go down. > I have tried refining in phenix and refmac both, but still the high R-free > problem persists. At this point I'm seeking help to know the possible > reasons for this high R-free. > > Thanks in advance! > > Jyotica > > > -- Jim Fairman, Ph D. Post-Doctoral Fellow National Institutes of Health - NIDDK Lab: 1-301-594-9229 E-mail: [log in to unmask] [log in to unmask]