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Does anyone know a commercial source of Cys-auxotroph strain of E. coli to prepare a doubly (Se-Cys + Se-Met) labeled protein? I would appreciate if you can share your experience if you have labeled your protein in this way to improve Se signal for phasing. Thanks, -Yogesh -- -------------------------------------------------- Yogesh K. Gupta, Ph.D. Dept of Structural & Chemical Biology Mount Sinai School of Medicine Icahn Medical Institute 1 Gustave L. Levy Place, Box 1677 New York, NY, USA 10029 Tel:+1 212-659-8639 --------------------------------------------------