To me, the first 3 pictures suggest that there is a faint greenish tinge in the little shapes (hopefully crystalline) there. I guess the idea of the Topaz chip is that you can take it as is to the x-ray beam or am I wrong?
Boaz
----- Original Message -----
From: Tri Ngo <
[log in to unmask]>
Date: Wednesday, September 29, 2010 10:50
Subject: [ccp4bb] The color of GFP crystals?
To:
[log in to unmask]> Dear CCP4 community,
>
> I wonder if anybody has experience about the color of GFP
> crystals. Is there
> any chance that the GFP crystal will become colorless? We are
> working on one
> membrane protein fused with GFP. Since we got some crystals
> which didn't
> show any color (Condition 01), I am not sure if I should go for
> optimization.
>
> Please check the link below for the image details:
> http://www.docstoc.com/docs/55950590/GFP-crystals
>
> Because of the limited amount of sample, we used Topaz system
> (screeningchip) to do the screening and there is no way to
> confirm these crystals are
> actually protein crystals. I noticed that the crystals are
> bigger day by
> day. Is it a good sign to confirm the protein crystals?
>
> Thank you very much for your helpful information!
>
> Best wishes,
>
> *
> TriNgo
>
> *
>
> Structural Biology Lab
>
> School of Medicine - Sungkyunkwan University
>
> Phone: 031-299-6150
>