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Not sure whether this is the kind of information you are looking for: The protein with PDB-ID 1ofc had a melting temperature of 37deg (from CD), which was supported by the fact that it did not express in E.coli at that temperature. At 20deg it expressed to about 60mg / (liter LB), could be concentrated to more than 100mg/ml, crystallised at room temperature and diffracted to 1.9A. The initial purification steps were done at 4deg, but I guess that's generally good advice anyhow. So maybe you don't need to worry too much, because stability is probably not the same as thermal stability. Cheers, Tim On Thu, Sep 23, 2010 at 11:28:20AM -0400, Raji Edayathumangalam wrote: > Hi Folks, > > Sorry for the pre-xtallo question; pre-xtallo right now, but hoping to > take my protein the xtallo way one of these days! > > I am currently performing Thermofluor assays with my protein and the > results show that the Tm is ~45C. I am looking for some examples of > proteins and their melting temperatures so that I can gauge where my > protein falls in the spectrum of unstable-to-stably folded. For example, > the melting temperature of some forms of lysozyme is 73.8C (very stable, > I suppose). > > Just need a sense for whether my protein is considered unstable or > somewhat stable. Please could you share some examples. > > Many thanks. > Raji > > ----------- > Raji Edayathumangalam > Joint Research Fellow > Harvard Medical School/ > Brigham and Women's Hospital > Brandeis University -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A