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Dear all, Anyone who have had success using the mosquito for micromatrix seeding? Which way is the optimal way of adding seed solution to sitting drops on an MRC plate? Is it better to add 100 nl protein + 10 nl seed solution + 100 nl reservoir solution or to add concentrated seed solution (1 microliter) directly to the reservoir solution and then pipett 100 nl protein + 100 nl reservoir solution. Best regards and thanks in advance, Maria Håkansson > > __________________________________________ > Maria Håkansson, Ph.D. > Senior Scientist, Max-lab, Lund University > Phone: +46 (0) 76 8585 706 > Fax: +46 (0) 46 222 47 10 > Ole Römers väg 1 (P.O. Box 188) > SE-221 00 Lund, Sweden > > Web address: www.maxlab.lu.se > Email: [log in to unmask] > __________________________________________