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Dear everyone,
Recently，we collected data of a native protein crystal on synchrotron in Shanghai. When we did with the original data with HKL2000, we found an unconversant phenomenon ,just as the picture in the enclosure, there were some grid indicated by arrows appearing on the low resolution areas.The crytal grew in 20%PEG3000，100mMTris-Cl，200mM Ca（OAc）2 ，and the cryo-protectant is 20% sucrose mixed with well buffer。Who can tell me the reasons it appear the grid ？because of the sucrose? I’ll appreciated for any explains and suggestion.
Best wishes
xingliangzhang