Hi Michele, >> Now my questions are: >> 1) Can I use SPM for simple MRI images and not for fMRI? Yes. For a complete anatomical image processing framework, search MedLine and the list archives at https://www.jiscmail.ac.uk/cgi-bin/webadmin?S1=spm for VBM. That'll keep you busy until about 2012 :) >> 2) can you give me instructions (from shell, from matlab, from spm) to follow to compare two MRI at two different times points of the same patient? That would be longitundinal VBM, see advice above. >> 3) can you indicate me some articols about the procedures mathematician at the base of any single algorithm of SPM (exctration, segmentation, ecc.)? what is the name of any algorithm (exctration, segmentation, ecc.)? SPM is heavily annotated in the code, and there is an online bibliography at http://www.fil.ion.ucl.ac.uk/spm/doc/biblio/ , the content of which will easily keep you busy for another 2 years :) Hope this helps, Marko -- ===================================================================== Marko Wilke (Dr.med./M.D.) [log in to unmask] Universitäts-Kinderklinik University Children's Hospital Abt. III (Neuropädiatrie) Dept. III (Pediatric neurology) Hoppe-Seyler-Str. 1, D - 72076 Tübingen Tel.: (+49) 07071 29-83416 Fax: (+49) 07071 29-5473 =====================================================================