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Hi Branislava, If the VOI is not generated with the option <adjust for main effect>, will that make a big impact on later analysis? Thanks! Lingling ________________________________ From: Branislava Curcic-Blake <[log in to unmask]> To: [log in to unmask] Sent: Thu, August 5, 2010 2:33:50 PM Subject: Re: [SPM] Eigenvariate Dear Maria, In order to get an option <adjust for main effect> you need to create an F-contrast of e.g main effect in your original SPM.mat. When creating a Volume of Interest (VOI) using eigenvariate button, the spm will calculate the eigen variate of the time courses of voxels that survive a certain (by you defined) contrast. Kind Regards Branislava