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Hello, I am trying to apply classification method to generate tracks from the PFC to basal ganglia ROI. I followed steps in Johansen-Berg et al., 2005 in Cerebral Cortex, "Functional-anatomical validation and individual variation of diffusion tractography-based segmentation of the human thalamus". In the paper, it said "For each cortical area, we thresholded and binarized individual subject results to include only those thalamic voxels with a connection probability > 25%." I wonder how this 25% is determined. I assume this threshold should be varied by the size of target mask. In my case, the target ROI is very small, 3mm sphere. What might be an appropriate threshold I should use? Thanks Best, Weidong Cai Dept of Psychology UCSD