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Yea, Parveen has actually asked about this here a year ago and I found this discussion quite useful indeed:

[log in to unmask]">[log in to unmask]

and I think we all get tons of these crystals especially in conditions with PEGs.
There are lots of things you can try and have been suggested, but I found this reference most helpful by far:

"A general protocol for the crystallization of membrane proteins for X-ray structural investigation."
http://www.ncbi.nlm.nih.gov/pubmed/19360018
(this is actually the paper in the link that Eric posted: http://kb.psi-structuralgenomics.org/update/2009/06/full/th_psisgkb.2009.25.html )

Best,
Matt

On Tue, Aug 31, 2010 at 12:53 PM, Parveen Goyal <[log in to unmask]> wrote:
Hi,

The picture looks like detergent crystals, specially DDM crystals. I have same crystals in many conditions.

With regards,
Parveen



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Matthew L.H. Chu, PhD
Postdoctoral Scholar - Weis Lab
Department of Structural Biology
Fairchild D143, MC 5126
Stanford School of Medicine
Stanford, CA 94305-5432
Lab: 650-724-3306
Alternative Email: [log in to unmask]
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