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As I'm now retired, I'm not certain what my old lab does now. However, in my days (and doesn't that make me feel old), we asked for control sample preferably from the doctor making the original request. If the control was within the reference range, we had more confidence that the batch of tubes, reagents etc was OK Regards Elliott ________________________________ From: Clinical biochemistry discussion list [[log in to unmask]] On Behalf Of COLLINS MICHAEL (RM1) Norfolk and Norwich University Hospital [[log in to unmask]] Sent: 13 August 2010 13:07 To: [log in to unmask] Subject: Re: Ammonia contamination - paediatric tubes One problem with ammonia assays is that lab water can quickly become contaminated. This can cause complications with manual dilutions unless a blank is run using water taken from the same source at the same time. You need to ensure that the “blank” solution is not itself contaminated by running a blank not placed in the paediatric tube. Mike Collins BMS3 Biochemistry Automation Norfolk & Norwich University Hospital England [log in to unmask] http://www.nnuh.nhs.uk/ ________________________________ From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Tennant Brian Sent: 13 August 2010 11:53 To: [log in to unmask] Subject: Ammonia contamination - paediatric tubes Dear All, We have recently had a problem with a batch of contaminated paediatric EDTA tubes when measuring ammonia. When we experienced problems previously, we ran a 'blank' sample from the same batch alongside each patient sample, but this practise was not continued routinely. I was just wondering if other labs did/ didn't always run blanks for ammonia, in routine practise? Those that do, what procedures do you follow? Thanks in advance for any help, Brian Brian Tennant Dept Clinical Biochemistry Mayday Healthcare NHS Trust 020 8401 3548 This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses but this should not be relied upon as a guarantee that the contents are virus free. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of the Norfolk and Norwich University Hospitals NHS Foundation Trust. The information contained in this e-mail may be subject to public disclosure under the Freedom of Information Act 2000. 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