A benefit of this is that it predicts your domains, makes sequence alignments with the similar proteins, and makes homology models which are sometimes good enough to start building with when you've phased the structure!

Another benefit is that the server runs "disopred", the disorder prediction server for you, and then aligns the output with the domain predictions. These domain predictions are then a good place to start when designing constructs...

On 29 July 2010 07:58, vikrant saa <[log in to unmask]> wrote:

I want to do cloning of a 40 Kd protein in pRSETA, and pGEX-KT vector. I don't have any idea about protein solubility, its multimeric form, stability and disorder etc. There is nothing known in the literature also. Is there any software that can predict these parameters, so that i can decide which domain i need to clone for soluble and stable protein purification.

Vikrant

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Junior Research Fellow (CSIR)

Lab No. 101, Dr. Varma Lab

Cancer Research Institute
Advance Centre for Treatment, Research and Eduction in Cancer (ACTREC)
Tata Memorial Hospital, Kharghar, Navi Mumbai-410210
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