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Tried with my protein and it says *very* difficult but I got crystals. Quoting Albert Guskov <[log in to unmask]>: > Hi Vikrant, > I guess Xtalpred server might be of interest for you. > check it at http://ffas.burnham.org/XtalPred-cgi/xtal.pl > Cheers, > *Albert GUSKOV (Dr) *| Research Fellow | Division of Structural & > Computational Biology | Nanyang Technological University > Proteos 7-01, Biopolis Drive 61, Singapore 138673 Tel: (65) 6586-9690 GMT+8h > | Cell: (65) 8366-2779 | Email: [log in to unmask] | Web: www.ntu.edu.sg > > 2010/7/29 vikrant saa <[log in to unmask]> > >> I want to do cloning of a 40 Kd protein in pRSETA, and pGEX-KT vector. I >> don't have any idea about protein solubility, its multimeric form, stability >> and disorder etc. There is nothing known in the literature also. Is there >> any software that can predict these parameters, so that i can decide which >> domain i need to clone for soluble and stable protein purification. >> *****Vikrant >> *** >> >> ------------------------------------------------------------------------------------------------------------------------ >> ***Junior Research Fellow (CSIR) * >> *Lab No. 101, Dr. Varma Lab* >> *Cancer Research Institute >> Advance Centre for Treatment, Research and Eduction in Cancer (ACTREC) >> Tata Memorial Hospital, Kharghar, Navi Mumbai-410210 * >> ##################################################################### >> >> >> >> > -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS Phone number: 02078828480