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Hi, I think the MAXCHN "errors" has been "fixed" in recent versions of CNS. You must be using CNS v1.0 or v1.1. So either you download and install a recent version (the latest is v1.3), or you do as the error message suggests: you edit your anneal.inp file, you locate "MAXCHN=" or "MAXCHN =" (or "maxchn=" or "maxchn =" - can't tell you which one since I have removed previous versions of CNS on my workstation) and you increase the figure after the = sign. I cannot remember if the topology error message is related to the problem with an insufficient number of chains set in the input file. HTH, Fred. Hussain Bhukyagps wrote: > Hi, > > I'm facing problem in refinement. > i have a pdb and it is showing the following error in the step of > annealing. > > > > > > -------------------------- Torsion Topology > ------------------------------ > ----- > ERROR: A tree has too many chains. > Please increase MAXCHN and re-run. > > %TORSION:TOPOLOGY error encountered: Fatal Topology Error > (CNS is in mode: SET ABORT=NORMal END) > ***************************************************** > ABORT mode will terminate program execution. > ***************************************************** >