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Thanks Ian. Good for David Bruns. My interest is in the possible ex vivo generation of D-lactate, in the presence of fluoride. It could be generated from methylglyoxal (see Annals 2010;47, page 269) itself potentially derived from the action of triose phosphate isomerase, during normal glycolytic metabolism. But if fluoride is a rapid inhibitor of enolase, that is not going to happen. Nick On 24 June 2010 14:19, ianholbrook <[log in to unmask]> wrote: > Nick > See > Clinical Chemistry 54: 930-932, 2008 > > http://www.clinchem.org/cgi/content/full/54/5/930 > > > Kind regards > Ian > -----Original Message----- > From: Clinical biochemistry discussion list > [mailto:[log in to unmask]] On Behalf Of Nick Miller > Sent: 24 June 2010 14:09 > To: [log in to unmask] > Subject: Re: FW: CSF analysis > > I know this is a widely held view, but can anyone prove to me that fluoride > (by which we mean 5.0 mg of sodium fluoride in a maximum of 2.0 ml of > sample) "stops lactate production immediately"? (see below) > > Nick Miller > London > > On 24 June 2010 11:08, Holbrook, Ian B <[log in to unmask]> wrote: >> >> ________________________________ >> From: Holbrook, Ian B >> Sent: 24 June 2010 11:07 >> To: 'Turner Helen (NHS Grampian)' >> Cc: 'Dina' >> Subject: RE: CSF analysis >> >> Dear Helen >> >> On behalf of the UK Specialist Advisory Group for EQA of CSF Proteins >> and Biochemistry we would ALWAYS recommend analysing CSF if sufficient >> sample is received. This is precious material that often cannot be > repeated. >> >> If a blood stained CSF sample has a protein level within the reference >> range then this is useful information to the clinician. If it is above >> the reference range then of course appropriate comments must be added >> to help in interpretation. If the results could have been affected by >> the state of the CSF, delays in transport, not protected from the >> light etc. then comments on interpretation will form part of the report. >> >> I would think that you could analyse CSF glucose in an unpreserved >> sample as long as this was done within an hour of collection. Fluoride >> stops lactate production immediately but does not stop glucose >> utilisation for an hour or so. There would probably be little >> difference, therefore, between preserved and unpreserved samples within an > hour of collection. >> >> Kind regards >> >> Ian >> >> >> Ian Holbrook >> >> Department of Clinical Biochemistry >> >> York Hospital >> >> Wigginton Road >> >> York >> >> YO31 8HE >> >> 01904 725786 >> >> >> >> ________________________________ >> From: Clinical biochemistry discussion list >> [mailto:[log in to unmask]] On Behalf Of Turner Helen >> (NHS >> Grampian) >> Sent: 22 June 2010 17:52 >> To: [log in to unmask] >> Subject: CSF analysis >> >> Dear readers, >> >> We are currently having a few disagreements regarding CSF analysis and >> hence I'm looking for some advice. >> >> 1- How to deal with blood stained samples for CSF protein, glucose, >> IgG, albumin and oligoclonal band analysis. Should these be reported >> as unsuitable, or analysed with a comment to say interpret with >> caution due to blood stained sample? In the case of oligoclonal bands- >> does the fact the sample is blood stained have an effect? >> >> 2- Tube type for CSF Glucose analysis. Does this have to be a Fl-Ox >> tube, or can a plain universal be used if received in the lab quickly > (<1hr). >> >> >> Thanks in advance >> >> Helen >> >> Clinical Biochemist >> Department of Clinical Biochemistry >> 1st Floor Link Building >> Aberdeen Royal Infirmary >> Foresterhill >> Aberdeen >> AB25 2ZD >> >> Tel: 01224 552831 >> >> ********************************************************************** >> ********************************************** >> >> This message may contain confidential information. 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Views expressed are those of the individual and they are > responsible for all message content. > ACB Web Site > http://www.acb.org.uk > Green Laboratories Work > http://www.laboratorymedicine.nhs.uk > List Archives > http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html > List Instructions (How to leave etc.) > http://www.jiscmail.ac.uk/ > > > > ******************************************************************************************************************** > > This message may contain confidential information. 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Views expressed are those of the individual and they are responsible for all message content. > ACB Web Site > http://www.acb.org.uk > Green Laboratories Work > http://www.laboratorymedicine.nhs.uk > List Archives > http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html > List Instructions (How to leave etc.) > http://www.jiscmail.ac.uk/ > ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/