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Dear MAgnus, From the error message it looks like all the time values are understood to be 0.0. That would clearly make the data set unfittable. Of ocurse the more interestingt question is how this situation arose, but hopefully this can give a clue. I am at home at teh moment, so others must take it from here. yours, Rasmus --------------------------------------------------------------------------- Dr. Rasmus H. Fogh Email: [log in to unmask] Dept. of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002 On Wed, 19 May 2010, Magnus Kjaergaard wrote: > Hello > > I'm using the "Analyzing Intensity changes" tool to analyze a set of > relaxation data. It has been working smoothly for a long time, but with > this particular data set it gives the following error message: > > Problem with fitting: method = 3, nIter = 1000, noise = 44752.1456529, x > = [0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0], y = [244499.921875, > 1193889.125, 624539.1875, 625447.9375, 616128.75, 479999.03125, > 400424.25, 354040.625, 316764.53125], xW = [0.0, 0.0, 0.0, 0.0, 0.0, > 0.0, 0.0, 0.0, 0.0], yW = [18067.869782831152, 44752.145652924628, > 88411.908721080879, 164042.48907585678, 93609.009951588741, > 50297.994252277269, 49875.262819463402, 31504.340190993345, > 73849.908842453093]: singular data > > What am I doing wrong? > > Magnus >